Bacteriophage – PHI X 174
The phi X bacteriophage is of interest because it was the first DNA-based genome to be sequenced. This ground-breaking work was done by Frederick Sanger who was twice awarded the Nobel Prize for Chemistry. In 2003 Craig Ventor’s team completely assembled the genome in vitro from synthesized oligonucleotides. This bacteriophage is a virus that attacks the Escherichia coli or E-Coli.
The Phi X is regularly used as a positve control in DNA sequencing experiments because of the extensive research on it as well as its relatively small genome size. The bacteriophage is a circular single-stranded DNA genome of 5386 nucleotides encoding 11 proteins of which only 8 are essential to viral morphogenesis.
The Phi X 174 comes from the Microviridae Family and the Microvirus Genus. (Species: ΦX174 phage)